Paf-Metabolic Enzymes and Paf-like Activity in L. Infantum and L. Major Promastigotes

In Vitro Activity of Cordia myxa Mucilage Extract Against Leishmania major and L. infantum Promastigotes

1Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran 2Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran 3Department of Dermatology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran 4Department of Pharmacy, Ahvaz Jundishapur University o...

Lysophosphatidylcholine and lyso-PAF display PAF-like activity derived from contaminating phospholipids.

Lysophosphatidylcholine is an abundant component of plasma and oxidized LDL that displays several biological activities, some of which may occur through the platelet-activating factor (PAF) receptor. We find that commercial lysophosphatidylcholine, its alkyl homolog (lyso-PAF), and PAF all induce inflammation in a murine model of pleurisy. Hydrolysis of PAF to lyso-PAF by recombinant PAF acetyl...

PAF-acetylhydrolase expressed during megakaryocyte differentiation inactivates PAF-like lipids.

Platelet activating factor (PAF) and PAF-like lipids induce inflammatory responses in target cells. These lipid mediators are inactivated by PAF-acetylhydrolase (PAF-AH). The PAF signaling system affects the growth of hematopoietic CD34(+) cells, but roles for PAF-AH in this process are unknown. Here, we investigated PAF-AH function during megakaryopoiesis and found that human CD34(+) cells acc...

in vitro activity of cordia myxa mucilage extract against leishmania major and l. infantum promastigotes

Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis.

Stimulated inflammatory cells synthesize platelet-activating factor (PAF), but lysates of these cells show little enhancement in PAF synthase activity. We show that human neutrophils contain intracellular plasma PAF acetylhydrolase (PLA2G7), an enzyme normally secreted by monocytes. The esterase inhibitors methyl arachidonoylfluorophosphonate (MAFP), its linoleoyl homolog, and Pefabloc inhibit ...